Standard Operating Procedures: A Video Training Series
Gene Expression Using Affymetrix U133 Plus 2.0 Arrays
Affymetrix developed the DNA Chip that has been used for numerous genomic analyses. The Stanford Genome Technology Center established an extensive collaboration with Affymetrix in 1990 shortly after it was founded that resulted in the development of DNA sequence tiling arrays and the detection of single nucleotide polymorphisms. This collaboration led to the first use of DNA microarrays for monitoring gene expression in 1995. Since that time, DNA microarrays have been used for quantifying gene expression for many organisms, including Human. This video was made to assist researchers and students who have been unable to obtain reproducible results because they were not following a standard operating procedure (SOP). Using the most common array for the quantification of Human gene expression, this video illustrates our protocol, which has been found to give very reproducible results. It can also be used to demonstrate proper lab skills for molecular biology. We believe that the best approach to teaching laboratory practice is through a professionally produced video of an excellent researcher conducting the protocol.
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