Standard Operating Procedures: A Video Training Series
Gene Expression Using Affymetrix U133 Plus 2.0 Arrays
“This training video was made because many researchers and students have been unable to obtain reproducible results because they were not using a standard operating procedure. Ronald W. Davis” [more]
To watch the videos in QuickTime, click on the "Play" links. We highly recommended to view these with high-speed internet connection. QuickTime 7 (download Mac/Windows) is required to view the movies.
This training video is also available on DVD.
Videos |
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Ctrl-Click to download | |
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Right-Click to download | ||
| 1. Introduction | Play | Download (12 MB) | |
| 2. cDNA Synthesis | |||
| a. Preparation of Total RNA | Play | Download (52 MB) | |
| b. Addition of T7 Primer | Play | Download (92 MB) | |
| c. First Strand Synthesis | Play | Download (85 MB) | |
| d. Second Strand Synthesis | Play | Download (79 MB) | |
| 3. cDNA Clean Up | Play | Download (277 MB) | |
| 4. IVT Set Up | Play | Download (87 MB) | |
| 5. cRNA Clean Up | Play | Download (263 MB) | |
| 6. Fragmentation and Hybridization | |||
| a. Fragmentation | Play | Download (75 MB) | |
| b. Hybridization | Play | Download (180 MB) | |
| 7. Washing Staining Scanning | |||
| a. Fluidics Station Prime | Play | Download (20 MB) | |
| b. Washing and Staining | Play | Download (56 MB) | |
| c. Scanning | Play | Download (32 MB) | |
| d. Data Export | Play | Download (8 MB) | |
Download the protocol files for this procedure.
