Standard Operating Procedures: A Video Training Series
Gene Expression Using Affymetrix U133 Plus 2.0 Arrays

This training video was made because many researchers and students have been unable to obtain reproducible results because they were not using a standard operating procedure. Ronald W. Davis [more]

To watch the videos in QuickTime, click on the "Play" links. We highly recommended to view these with high-speed internet connection. QuickTime 7 (download Mac/Windows) is required to view the movies.
This training video is also available on DVD.

Videos

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1. Introduction Play Download (12 MB)
2. cDNA Synthesis    
a. Preparation of Total RNA Play Download (52 MB)
b. Addition of T7 Primer Play Download (92 MB)
c. First Strand Synthesis Play Download (85 MB)
d. Second Strand Synthesis Play Download (79 MB)
3. cDNA Clean Up Play Download (277 MB)
4. IVT Set Up Play Download (87 MB)
5. cRNA Clean Up Play Download (263 MB)
6. Fragmentation and Hybridization    
a. Fragmentation Play Download (75 MB)
b. Hybridization Play Download (180 MB)
7. Washing Staining Scanning    
a. Fluidics Station Prime Play Download (20 MB)
b. Washing and Staining Play Download (56 MB)
c. Scanning Play Download (32 MB)
d. Data Export Play Download (8 MB)

Download the protocol files for this procedure.