Deletion Page Home

Databases and Datasets

FAQs

Deletion Strains Available

References

Protocols &Technical Information

Useful Sites & Links

Consortium Members' Addresses

Yeast Deletion Database (consortium members only)

 

References

Sites & Links

Yeast Deletion Database (consortium members only)

Functional Characterization of the Saccharomyces cerevisiae Genome by Gene Deletion and Parallel Analysis

Elizabeth A. Winzeler* Daniel D. Shoemaker*, Anna Astromoff*, Hong Liang*, Keith Anderson, Bruno Andre, Rhonda Bangham, Rocio Benito, Jef D. Boeke, Howard Bussey, Angela M. Chu, Carla Connelly, Karen Davis, Fred Dietrich, Sally Whelen Dow, Mohamed El Bakkoury, Françoise Foury,Stephen H. Friend, Erik Gentalen, Guri Giaever, Johannes H. Hegemann, Ted Jones, Michael Laub, Hong Liao, Nicole Liebundguth, David J. Lockhart, Anca Lucau-Danila, Marc Lussier, Nasiha M'Rabet, Patrice Menard, Michael Mittmann, Li Ni, Chai Pai, Corinne Rebischung, Jose L. Revuelta, Linda Riles, Christopher J. Roberts, Petra Ross-MacDonald, Bart Scherens, Michael Snyder, Sharon Sookhai-Mahadeo, Reginald K. Storms, Steeve Véronneau, Marleen Voet, Guido Volckaert, Teresa R. Ward, Robert Wysocki, Grace S. Yen, Kexin Yu, Katja Zimmermann, Peter Philippsen, Mark Johnston, and Ronald W. Davis (1)

* These authors contributed equally to this work.

(1) Department of Biochemistry, Beckman Center, Stanford University Medical Center, Stanford, CA 94305, USA

The functions of most open reading frames (ORFs) identified in genome-sequencing projects is unknown. New, whole genome approaches are required to systematically determine their function. A total of 6925 Saccharomyces cerevisiae strains were constructed, by a high-thoughput strategy, each with a precise deletion of one of 2026 ORFs (more than one-third of the ORFs in the genome). Of the deleted ORFs, 17 percent were essential for viability in rich medium. The phenotypes of more than 500 deletion strains were assayed in parallel. Of the deletion strains, 40 percent showed quantitative growth defects in either rich or minimal medium.

Science. 285 : 901-906 (1999)

For more information on the figures from the Science paper see:

Searchable Database for Figure 2 (essential genes)


Searchable Database for Figure 3 (phenotypic analysis results)

 

To download the above databases as tab delimited text tables
onto your computer, use "SAVE AS. . . " in your web browser.

click here for Figure2 (essential genes)

click here for Figure 3 (phenotypic analysis results)

 

 

last updated 02.20.02